1/3/2023 0 Comments Mouse hippocampus anatomy![]() ![]() Here, a detailed protocol for a successful preparation of vibratome-cut horizontal hippocampal slices of mouse brains is provided. Moreover, different cutting planes can be applied for vibratome brain slice preparations. Brain slice preparations with a vibratome allow a better preservation of the tissue structure and guarantee a sufficient oxygen supply during slicing, which present advantages over the traditional use of a tissue chopper. There exist several different ways of slice preparations. Therefore, brain slices represent an ideal model system to assess protein functions, to investigate pathophysiological processes involved in neurological disorders as well as for drug discovery purposes. Hippocampal brain slices can be used for several different applications, including electrophysiological recordings, light microscopic measurements as well as several molecular biological and histochemical techniques. Brain slices are a frequently used methodology when exploring neurophysiological functions of the hippocampus. This connectivity is accomplished via different informational flows in the form of fiber pathways. The hippocampus receives a high degree of intra- and inter-connectivity, securing a proper communication with internal and external brain structures. The hippocampus is a highly organized structure in the brain that is a part of the limbic system and is involved in memory formation and consolidation as well as the manifestation of severe brain disorders, including Alzheimer's disease and epilepsy. ![]()
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